Journal: Oncology Reports

Article Title: Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma

doi: 10.3892/or.2020.7510

Figure Lengend Snippet: Mechanism study of KIF15 KD in glioma. (A) Human apoptosis antibody array analysis was performed and analyzed in U87 MG cells with or without KIF15 KD. (B) The protein expression levels of Bax, p21 and Survivin in U251 cells were detected by western blot analysis. (C) The expression levels of Bax, p21 and Survivin were detected by IHC in tumor sections of a mouse xenograft model (magnification, ×200). (D) The expression levels of MEK1/2, p-MEK1/2, ERK1/2, p-ERK1/2 were detected by western blot analysis in U87 MG and U251 cells with or without KIF15 KD. *P<0.05, **P<0.01, ***P<0.001. KIF15, kinesin-12; KD, knockdown.

Article Snippet: Ki-67 antibody (1:100; cat. no. ab16667; Abcam), Bax antibody (1:250; cat. no. ab32503; Abcam), p21 antibody (1:50; cat. no. BM3990; BOSTER) and Survivin antibody (1:100; cat. no. ab469; Abcam) were used in the immunohistochemistry analysis of tumor sections removed from mice models.

Techniques: Ab Array, Expressing, Western Blot, Knockdown

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells

doi: 10.1155/2018/3853409

Figure Lengend Snippet: PSD-A induces mitochondrial dysfunctions and ER stress in A549 cells. (a) Cells were treated with indicated concentrations of PSD-A for 24 h. Cell lysates were prepared and subjected to Western blot for the expressions of Bax, Bcl-2, Bcl-xl, Xiap, and survivin. GAPDH was used as loading control. (b) Cells were treated with PSD-A for 24 h in the presence or absence of BAPTA-AM (10 μ M), and MMP was determined by staining the cells with JC-1 according to the product's instructions. Data are expressed as mean ± SEM ( n = 3). Columns not sharing the same superscript letters differ significantly ( P < 0.05). (c, d) Cells were exposed to the indicated concentrations of PSD-A for 24 h. Total cell lysates were extracted, and expressions of p-eIF2 α , eIF2 α , CHOP, ATF4, GRP90, and GRP78 were measured by Western blot analyses. (e) Cells were treated with PSD-A for 24 h, and activity of caspase-4 was measured according to the kit's instructions. PSD-A increased the activity of caspase-4. Data are expressed as mean ± SEM ( n = 3). Columns not sharing the same superscript letters differ significantly ( P < 0.05).

Article Snippet: The primary antibodies for cleaved caspases (3 and 9), cleaved PARP, p-STAT3 (Tyr705), STAT3, p-SRC, and SRC were obtained from Cell Signaling Technology (Beverly, MA) while primary antibodies for Bax, Bcl-2, Bcl-xl, Xiap, survivin, ATF4, eIF2 α , GRP78, GRP98, GAPDH, SHP-1, SHP-2, PTEN, and TrxR1 were obtained from Proteintech (Wuhan, China).

Techniques: Western Blot, Control, Staining, Activity Assay